Pengisi suara bahasa Jepun

• Shirou Gou / Red One: Ryosuke Sakamoto
• Shingo Takasugi / Green Two: Takahiko Ota (Diperankan sebagai Naoto Ota)
• Ryuta Nanbara / Blue Three: Akito Osuga
• Mika Koizumi / Yellow Four: Yuki Yajima (episode 1-10)
• Jun Yabuki / Yellow Four: Sumiko Tanaka (episode 11-51)
• Hikaru Katsuragi / Pink Five: Michiko Makino
• Doktor Shibata: Tadao Nakamaru
• Hideo Kageyama / Doctor Man: Munemaru Kouda
• Mason: Hirohisa Nakata
• Farrah: Yoko Asuka
• Farrah Cat: Yukari Oshima
• Monster: Strong Kongo
• Bio Hunter Silver: Jiro Okamoto
• Mettzler: Hiroshi Izawa
• Narator: Ichiro Murakoshi

Pengisi suara bahasa Inggris untuk versi Filipino

• Shirou Gou (Kenny) / Red One: Henry Gallardo
• Shingo Takasugi (Sammy) / Green Two: Vincent Galang
• Ryouta Nanbara (Franky) / Blue Three: Jake Chua
• Mika Koizumi (Casey) / Yellow Four: Nerrisa Coronel
• Jun Yabuki (June) / Yellow Four: Jenniffer Chua
• Hikaru Katsuragi (Kimberly) / Pink Five: Antoinette Luciano
• Mason: Charlie Yu
• Farrah: Marie Anne Galang
• Farrah Cat: Angie Almodiente
• Doctor Man: Rudy Wingfield